|
KMID : 0861020120270020053
|
|
Korea Journal of Herbology
2012 Volume.27 No. 2 p.53 ~ p.59
|
|
|
Ethyl acetate fraction of GGEx18 modulates fatty acid β-oxidizing enzymes
|
|
Joo Byung-Soo
Lee Hee-Young
Lee Hye-Rim
Yoon Mi-Jung
Seo Bu-Il
Kim Bum-Hoi
Shin Soon-Shik
|
|
|
Abstract
|
|
|
Objectives: This study was undertaken to investigate the effects of the GGEx18 ethyl acetate fraction (EF) on
lipid accumulation and gene expression of fatty acid-oxidizing enzymes using 3T3-L1 adipocytes, C2C12
skeletal muscle cells, and NMu2Li liver cells.
Methods: PPARα, AMPK and UCPs transactivation was examined in NMu2Li hepatocytes, C2C12 myocytes, and
3T3-L1 preadipocytes using transient transfection assays.
Results: 1. Compared with control, EF significantly increased the mRNA expression of VLCAD in 3T3-L1
adipocytes.
2. Compared with control, EF (0.1 ㎍/ml) significantly inhibited lipid accumulation in 3T3-L1 adipocytes.
3. EF significantly increased the mRNA expression of AMPKα1, AMPKα2 and PPARα in C2C12 skeletal muscle
cells compared with control.
4. EF significantly increased the mRNA expression of genes involved in fatty acid β-oxidation, such as
thiolase, MCAD, and CPT-1 in C2C12 skeletal muscle cells compared with control.
5. EF significantly increased the mRNA expression of UCP2 involved in energy expenditure in C2C12 skeletal
muscle cells compared with control.
6. Compared with control, EF (10 ㎍/ml) significantly inhibited lipid accumulation in C2C12 skeletal muscle
cells.
7. EF (10 ㎍/ml) significantly increased the mRNA expression of ACOX, HD, VLCAD and MCAD in NMu2Li liver
cells compared with control.
Conclusions: These results suggest that EF may prevent obesity by increasing the mRNA expression of
mitochondrial fatty acid β-oxidizing enzymes in 3T3-L1 adipocytes, by not only regulating the fatty acid
oxidation through activation of AMPK and PPARα, but also increasing the UCP2 mRNA expression in C2C12
skeletal muscle cells, and by stimulating the mRNA expression of fatty acid-oxidizing enzymes in NMu2Li liver
cells.
|
|
|
KEYWORD
|
|
|
Gyeongshingangjeehwan18, ethyl acetate fraction, fatty acid -oxidation, obesity
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|
|